@prefix ocrer: <http://purl.org/net/OCRe/research.owl#> .
@prefix owl:   <http://www.w3.org/2002/07/owl#> .
@prefix scires: <http://vivoweb.org/ontology/scientific-research#> .
@prefix xsd:   <http://www.w3.org/2001/XMLSchema#> .
@prefix skos:  <http://www.w3.org/2004/02/skos/core#> .
@prefix rdfs:  <http://www.w3.org/2000/01/rdf-schema#> .
@prefix ocresd: <http://purl.org/net/OCRe/study_design.owl#> .
@prefix swo:   <http://www.ebi.ac.uk/efo/swo/> .
@prefix cito:  <http://purl.org/spar/cito/> .
@prefix geo:   <http://aims.fao.org/aos/geopolitical.owl#> .
@prefix ocresst: <http://purl.org/net/OCRe/statistics.owl#> .
@prefix dcterms: <http://purl.org/dc/terms/> .
@prefix vivo:  <http://vivoweb.org/ontology/core#> .
@prefix event: <http://purl.org/NET/c4dm/event.owl#> .
@prefix vann:  <http://purl.org/vocab/vann/> .
@prefix foaf:  <http://xmlns.com/foaf/0.1/> .
@prefix c4o:   <http://purl.org/spar/c4o/> .
@prefix fabio: <http://purl.org/spar/fabio/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix thkoeln: <http://cris.nrw/hisinone#> .
@prefix vitro: <http://vitro.mannlib.cornell.edu/ns/vitro/0.7#> .
@prefix vitro-public: <http://vitro.mannlib.cornell.edu/ns/vitro/public#> .
@prefix rdf:   <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix ocresp: <http://purl.org/net/OCRe/study_protocol.owl#> .
@prefix bibo:  <http://purl.org/ontology/bibo/> .
@prefix obo:   <http://purl.obolibrary.org/obo/> .
@prefix ro:    <http://purl.obolibrary.org/obo/ro.owl#> .

obo:BFO_0000031  a  owl:Class ;
        rdfs:label  "Bedingter Kontinuant"@de-DE , "Generically Dependent Continuant"@en-US , "Generically Dependent Continuant"@en .

<https://fis.th-koeln.de/vivo/individual/FF_001>
        <http://cris.nrw/hisinone/istForschungsfeldvon>
                <https://fis.th-koeln.de/vivo/individual/publ_17083> .

<https://fis.th-koeln.de/vivo/individual/aut_17083-3>
        a             obo:BFO_0000001 , obo:BFO_0000020 , vivo:Authorship , vivo:Relationship , obo:BFO_0000002 , owl:Thing ;
        vivo:relates  <https://fis.th-koeln.de/vivo/individual/publ_17083> .

<http://cris.nrw/hisinone/OpenAccess>
        a           <http://cris.nrw/hisinone/Zugangsrecht> , owl:NamedIndividual , owl:Thing ;
        rdfs:label  "Open Access"@en-US , "Open Access"@de-DE .

obo:BFO_0000002  a  owl:Class ;
        rdfs:label  "Kontinuant"@de-DE , "Continuant"@en-US , "Continuant"@en .

<https://fis.th-koeln.de/vivo/individual/10000159>
        a           thkoeln:Organisationseinheit , foaf:Agent , obo:BFO_0000001 , obo:BFO_0000004 , obo:BFO_0000002 , foaf:Organization , owl:Thing ;
        rdfs:label  "Fakultät für Angewandte Naturwissenschaften" ;
        <http://cris.nrw/hisinone/istOrganisationseinheitVon>
                <https://fis.th-koeln.de/vivo/individual/publ_17083> .

<https://fis.th-koeln.de/vivo/individual/aut_17083-2>
        a             obo:BFO_0000001 , obo:BFO_0000020 , vivo:Authorship , vivo:Relationship , obo:BFO_0000002 , owl:Thing ;
        vivo:relates  <https://fis.th-koeln.de/vivo/individual/publ_17083> .

thkoeln:Artikel  a  owl:Class ;
        rdfs:label  "Artikel"@de-DE , "Article"@en-US .

<https://fis.th-koeln.de/vivo/individual/publ_17083>
        a                         obo:IAO_0000030 , bibo:Document , obo:BFO_0000001 , thkoeln:Publikation , foaf:Document , obo:BFO_0000002 , thkoeln:Artikel , thkoeln:Journalartikel , owl:Thing , obo:BFO_0000031 ;
        rdfs:label                "RDF description of Transposon Vector-mediated Stable Gene Transfer for the Accelerated Establishment of Recombinant Mammalian Cell Pools Allowing for High-yield Production of Biologics - https://fis.th-koeln.de/vivo/individual/publ_17083" , "Transposon Vector-mediated Stable Gene Transfer for the Accelerated Establishment of Recombinant Mammalian Cell Pools Allowing for High-yield Production of Biologics" ;
        thkoeln:URL               "https://europepmc.org/articles/PMC7275939" ;
        thkoeln:dokumententyp     "Wissenschaftlicher Artikel" ;
        thkoeln:peerReviewed      "true" ;
        thkoeln:sprache           "englisch" ;
        thkoeln:status            "validiert" ;
        <http://cris.nrw/hisinone/hatForschungsfeld>
                <https://fis.th-koeln.de/vivo/individual/FF_001> ;
        <http://cris.nrw/hisinone/hatOrganisationseinheit>
                <https://fis.th-koeln.de/vivo/individual/10000159> ;
        <http://cris.nrw/hisinone/hatRessourceart>
                <http://cris.nrw/hisinone/RessourceText> ;
        <http://cris.nrw/hisinone/hatZitat>
                <https://fis.th-koeln.de/vivo/individual/cite_17083-0> , <https://fis.th-koeln.de/vivo/individual/cite_17083-1> , <https://fis.th-koeln.de/vivo/individual/cite_17083-2> ;
        <http://cris.nrw/hisinone/hatZugangsrecht>
                <http://cris.nrw/hisinone/OpenAccess> ;
        <http://purl.org/dc/elements/1.1/date>
                "2026-04-04T00:09:30"^^xsd:dateTime ;
        <http://purl.org/dc/elements/1.1/publisher>
                <https://fis.th-koeln.de/vivo> ;
        <http://purl.org/dc/elements/1.1/rights>
                <https://fis.th-koeln.de/vivo/termsOfUse> ;
        bibo:abstract             "Stable recombinant mammalian cells are of growing importance in pharmaceutical biotechnology production scenarios for biologics such as monoclonal antibodies, growth and blood factors, cytokines and subunit vaccines. However, the establishment of recombinant producer cells using classical stable transfection of plasmid DNA is hampered by low stable gene transfer efficiencies. Consequently, subsequent selection of transgenic cells and the screening of clonal cell populations are time- and thus cost-intensive. To overcome these limitations, expression cassettes were embedded into transposon-derived donor vectors. Upon the co-transfection with transposase-encoding constructs, elevated vector copy numbers stably integrated into the genomes of the host cells are readily achieved facilitating under stringent selection pressure the establishment of cell pools characterized by sustained and high-yield recombinant protein production. Here, we discuss some aspects of transposon vector technologies, which render these vectors promising candidates for their further utilization in the production of biologics." ;
        bibo:doi                  "10.1007/s10529-020-02889-y" ;
        bibo:issue                "7" ;
        bibo:pageEnd              "1112" ;
        bibo:pageStart            "1103" ;
        bibo:volume               "42" ;
        vitro:mostSpecificType    thkoeln:Journalartikel ;
        vivo:dateTimeValue        <http://cris.nrw/date2020> ;
        vivo:freetextKeyword      "Sleeping Beauty" , "Cell Line" , "Genetic Vectors" , "Protein Production" , "Animals" , "PiggyBac" , "DNA Transposable Elements" , "Transposon Vector" , "Biological Products" , "Humans" , "Mammalian Cells" , "Recombinant Proteins" , "Transfection" , "Mammals" ;
        vivo:hasPublicationVenue  <https://fis.th-koeln.de/vivo/individual/journal_3097> ;
        vivo:relatedBy            <https://fis.th-koeln.de/vivo/individual/aut_17083-3> , <https://fis.th-koeln.de/vivo/individual/aut_17083-2> , <https://fis.th-koeln.de/vivo/individual/aut_17083-1> .

obo:BFO_0000001  a  owl:Class ;
        rdfs:label  "Entität"@de-DE , "Entity"@en-US , "Entity"@en .

<https://fis.th-koeln.de/vivo/individual/aut_17083-1>
        a             obo:BFO_0000001 , obo:BFO_0000020 , vivo:Authorship , vivo:Relationship , obo:BFO_0000002 , owl:Thing ;
        vivo:relates  <https://fis.th-koeln.de/vivo/individual/publ_17083> .

<http://cris.nrw/hisinone/RessourceText>
        a           owl:NamedIndividual , <http://cris.nrw/hisinone/ArtderRessource> , owl:Thing ;
        rdfs:label  "Text"@de-DE , "Text"@en-US .

bibo:Document  a    owl:Class ;
        rdfs:label  "Dokument"@de-DE , "Document"@en-US , "Document"@en .

<https://fis.th-koeln.de/vivo/individual/cite_17083-2>
        a       thkoeln:Zitat , owl:Thing .

owl:Thing  a    owl:Class .

thkoeln:Journalartikel
        a           owl:Class ;
        rdfs:label  "Journal Article"@en-US , "Journalartikel"@de-DE .

<https://fis.th-koeln.de/vivo/individual/journal_3097>
        a                         bibo:Collection , thkoeln:Zeitschrift , obo:BFO_0000031 , bibo:Journal , bibo:Periodical , obo:BFO_0000001 , owl:Thing , obo:BFO_0000002 , obo:IAO_0000030 ;
        rdfs:label                "Biotechnology letters" ;
        vivo:publicationVenueFor  <https://fis.th-koeln.de/vivo/individual/publ_17083> .

<https://fis.th-koeln.de/vivo/individual/cite_17083-1>
        a       thkoeln:Zitat , owl:Thing .

obo:IAO_0000030  a  owl:Class ;
        rdfs:label  "Entität für Informationsinhalt"@de-DE , "Information Content Entity"@en-US , "Information Content Entity"@en .

<http://cris.nrw/date2020>
        a       obo:BFO_0000003 , obo:BFO_0000008 , obo:BFO_0000001 , vivo:DateTimeValue , obo:BFO_0000148 , owl:Thing .

<https://fis.th-koeln.de/vivo/individual/cite_17083-0>
        a       thkoeln:Zitat , owl:Thing .

thkoeln:Publikation  a  owl:Class ;
        rdfs:label  "Publikationen"@de-DE , "Publications"@en-US .