Journalartikel - Wissenschaftlicher Artikel | 2021 | Peer reviewed

Reprograming of sRNA Target Specificity by the Leader Peptide peTrpL in Response to Antibiotic Exposure

Autor*innen

Melior, Hendrik ; Li, Siqi ; Stötzel, Maximilian ; Maaß, Sandra ; Schütz, Rubina ; Azarderakhsh, Saina ; Shevkoplias, Aleksei ; Barth-Weber, Susanne ; Baumgardt, Kathrin ; Ziebuhr, John ; Förstner, Konrad ; Chervontseva, Zoe ; Becher, Dörte ; Evguenieva-Hackenberg, Elena

Zeitschrift

Nucleic acids research

DOI

10.1093/nar/gkab093

URL

https://academic.oup.com/nar/article-pdf/49/5/2894/36627482/gkab093.pdf

Weitere Details

Sprache

englisch

Darstellungsform

Text

Band

Ausgabe

Seitenbereich

2894 - 2915

Trans-acting regulatory RNAs have the capacity to base pair with more mRNAs than generally detected under defined conditions, raising the possibility that sRNA target specificities vary depending on the specific metabolic or environmental conditions. In Sinorhizobium meliloti, the sRNA rnTrpL is derived from a tryptophan (Trp) transcription attenuator located upstream of the Trp biosynthesis gene trpE(G). The sRNA rnTrpL contains a small ORF, trpL, encoding the 14-aa leader peptide peTrpL. If Trp is available, efficient trpL translation causes transcription termination and liberation of rnTrpL, which subsequently acts to downregulate the trpDC operon, while peTrpL is known to have a Trp-independent role in posttranscriptional regulation of antibiotic resistance mechanisms. Here, we show that tetracycline (Tc) causes rnTrpL accumulation independently of Trp availability. In the presence of Tc, rnTrpL and peTrpL act collectively to destabilize rplUrpmA mRNA encoding ribosomal proteins L21 and L27. The three molecules, rnTrpL, peTrpL, and rplUrpmA mRNA, form an antibiotic-dependent ribonucleoprotein complex (ARNP). In vitro reconstitution of this ARNP in the presence of competing trpD and rplU transcripts revealed that peTrpL and Tc cause a shift of rnTrpL specificity towards rplU, suggesting that sRNA target prioritization may be readjusted in response to changing environmental conditions.

Zitierstile

Melior, H., Li, S., Stötzel, M., Maaß, S., Schütz, R., Azarderakhsh, S., … Evguenieva-Hackenberg, E. (2021). Reprograming of sRNA Target Specificity by the Leader Peptide peTrpL in Response to Antibiotic Exposure. Nucleic Acids Research, 49(5), 2894–2915. doi:10.1093/nar/gkab093

In die Zwischenablage kopieren

Melior, H., Li, S., Stötzel, M., Maaß, S., Schütz, R., Azarderakhsh, S., Shevkoplias, A., Barth-Weber, S., Baumgardt, K., Ziebuhr, J., Förstner, K., Chervontseva, Z., Becher, D. and Evguenieva-Hackenberg, E., 2021. Reprograming of sRNA Target Specificity by the Leader Peptide peTrpL in Response to Antibiotic Exposure. Nucleic acids research, [online] 49(5), pp.2894–2915. Available at: <https://academic.oup.com/nar/article-pdf/49/5/2894/36627482/gkab093.pdf>.

In die Zwischenablage kopieren

Melior, Hendrik u. a. 2021. Reprograming of sRNA Target Specificity by the Leader Peptide peTrpL in Response to Antibiotic Exposure. Nucleic acids research 49, 5, 2894–2915. https://academic.oup.com/nar/article-pdf/49/5/2894/36627482/gkab093.pdf.

American Psychological Association 6th edition (DOI)

Anglia Ruskin University - Harvard

Gesellschaft für Bildung und Forschung in Europa - Harvard