{
  "@graph" : [ {
    "@id" : "http://cris.nrw/date2023",
    "@type" : [ "obo:BFO_0000003", "obo:BFO_0000008", "vivo:DateTimeValue", "obo:BFO_0000148" ],
    "rdf:type" : [ {
      "@id" : "obo:BFO_0000001"
    }, {
      "@id" : "owl:Thing"
    } ]
  }, {
    "@id" : "thkoeln:Artikel",
    "@type" : "owl:Class",
    "label" : [ {
      "@language" : "de-de",
      "@value" : "Artikel"
    }, {
      "@language" : "en-us",
      "@value" : "Article"
    } ]
  }, {
    "@id" : "thkoeln:Journalartikel",
    "@type" : "owl:Class",
    "label" : [ {
      "@language" : "en-us",
      "@value" : "Journal Article"
    }, {
      "@language" : "de-de",
      "@value" : "Journalartikel"
    } ]
  }, {
    "@id" : "thkoeln:Publikation",
    "@type" : "owl:Class",
    "label" : [ {
      "@language" : "de-de",
      "@value" : "Publikationen"
    }, {
      "@language" : "en-us",
      "@value" : "Publications"
    } ]
  }, {
    "@id" : "http://cris.nrw/hisinone/OpenAccess",
    "@type" : [ "http://cris.nrw/hisinone/Zugangsrecht", "owl:NamedIndividual" ],
    "rdf:type" : {
      "@id" : "owl:Thing"
    },
    "label" : [ {
      "@language" : "en-us",
      "@value" : "Open Access"
    }, {
      "@language" : "de-de",
      "@value" : "Open Access"
    } ]
  }, {
    "@id" : "http://cris.nrw/hisinone/RessourceText",
    "@type" : [ "owl:NamedIndividual", "http://cris.nrw/hisinone/ArtderRessource" ],
    "rdf:type" : {
      "@id" : "owl:Thing"
    },
    "label" : [ {
      "@language" : "de-de",
      "@value" : "Text"
    }, {
      "@language" : "en-us",
      "@value" : "Text"
    } ]
  }, {
    "@id" : "obo:BFO_0000001",
    "@type" : "owl:Class",
    "label" : [ {
      "@language" : "de-de",
      "@value" : "Entität"
    }, {
      "@language" : "en-us",
      "@value" : "Entity"
    }, {
      "@language" : "en",
      "@value" : "Entity"
    } ]
  }, {
    "@id" : "obo:BFO_0000002",
    "@type" : "owl:Class",
    "label" : [ {
      "@language" : "de-de",
      "@value" : "Kontinuant"
    }, {
      "@language" : "en-us",
      "@value" : "Continuant"
    }, {
      "@language" : "en",
      "@value" : "Continuant"
    } ]
  }, {
    "@id" : "obo:BFO_0000031",
    "@type" : "owl:Class",
    "label" : [ {
      "@language" : "de-de",
      "@value" : "Bedingter Kontinuant"
    }, {
      "@language" : "en-us",
      "@value" : "Generically Dependent Continuant"
    }, {
      "@language" : "en",
      "@value" : "Generically Dependent Continuant"
    } ]
  }, {
    "@id" : "obo:IAO_0000030",
    "@type" : "owl:Class",
    "label" : [ {
      "@language" : "de-de",
      "@value" : "Entität für Informationsinhalt"
    }, {
      "@language" : "en-us",
      "@value" : "Information Content Entity"
    }, {
      "@language" : "en",
      "@value" : "Information Content Entity"
    } ]
  }, {
    "@id" : "bibo:Document",
    "@type" : "owl:Class",
    "label" : [ {
      "@language" : "de-de",
      "@value" : "Dokument"
    }, {
      "@language" : "en-us",
      "@value" : "Document"
    }, {
      "@language" : "en",
      "@value" : "Document"
    } ]
  }, {
    "@id" : "owl:Thing",
    "@type" : "owl:Class"
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/10000159",
    "@type" : [ "thkoeln:Organisationseinheit", "foaf:Agent", "obo:BFO_0000004", "foaf:Organization" ],
    "istOrganisationseinheitVon" : "https://fis.th-koeln.de/vivo/individual/publ_13676",
    "rdf:type" : [ {
      "@id" : "obo:BFO_0000001"
    }, {
      "@id" : "obo:BFO_0000002"
    }, {
      "@id" : "owl:Thing"
    } ],
    "label" : "Fakultät für Angewandte Naturwissenschaften"
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/FF_001",
    "istForschungsfeldvon" : "https://fis.th-koeln.de/vivo/individual/publ_13676"
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/aut_13676-1",
    "@type" : [ "obo:BFO_0000020", "vivo:Authorship", "vivo:Relationship" ],
    "relates" : "https://fis.th-koeln.de/vivo/individual/publ_13676",
    "rdf:type" : [ {
      "@id" : "obo:BFO_0000001"
    }, {
      "@id" : "obo:BFO_0000002"
    }, {
      "@id" : "owl:Thing"
    } ]
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/aut_13676-2",
    "@type" : [ "obo:BFO_0000020", "vivo:Authorship", "vivo:Relationship" ],
    "relates" : "https://fis.th-koeln.de/vivo/individual/publ_13676",
    "rdf:type" : [ {
      "@id" : "obo:BFO_0000001"
    }, {
      "@id" : "obo:BFO_0000002"
    }, {
      "@id" : "owl:Thing"
    } ]
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/aut_13676-3",
    "@type" : [ "obo:BFO_0000020", "vivo:Authorship", "vivo:Relationship" ],
    "relates" : "https://fis.th-koeln.de/vivo/individual/publ_13676",
    "rdf:type" : [ {
      "@id" : "obo:BFO_0000001"
    }, {
      "@id" : "obo:BFO_0000002"
    }, {
      "@id" : "owl:Thing"
    } ]
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/aut_13676-4",
    "@type" : [ "obo:BFO_0000020", "vivo:Authorship", "vivo:Relationship" ],
    "relates" : "https://fis.th-koeln.de/vivo/individual/publ_13676",
    "rdf:type" : [ {
      "@id" : "obo:BFO_0000001"
    }, {
      "@id" : "obo:BFO_0000002"
    }, {
      "@id" : "owl:Thing"
    } ]
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/aut_13676-5",
    "@type" : [ "obo:BFO_0000020", "vivo:Authorship", "vivo:Relationship" ],
    "relates" : "https://fis.th-koeln.de/vivo/individual/publ_13676",
    "rdf:type" : [ {
      "@id" : "obo:BFO_0000001"
    }, {
      "@id" : "obo:BFO_0000002"
    }, {
      "@id" : "owl:Thing"
    } ]
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/aut_13676-6",
    "@type" : [ "obo:BFO_0000020", "vivo:Authorship", "vivo:Relationship" ],
    "relates" : "https://fis.th-koeln.de/vivo/individual/publ_13676",
    "rdf:type" : [ {
      "@id" : "obo:BFO_0000001"
    }, {
      "@id" : "obo:BFO_0000002"
    }, {
      "@id" : "owl:Thing"
    } ]
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/aut_13676-7",
    "@type" : [ "obo:BFO_0000020", "vivo:Authorship", "vivo:Relationship" ],
    "relates" : "https://fis.th-koeln.de/vivo/individual/publ_13676",
    "rdf:type" : [ {
      "@id" : "obo:BFO_0000001"
    }, {
      "@id" : "obo:BFO_0000002"
    }, {
      "@id" : "owl:Thing"
    } ]
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/aut_13676-8",
    "@type" : [ "obo:BFO_0000020", "vivo:Authorship", "vivo:Relationship" ],
    "relates" : "https://fis.th-koeln.de/vivo/individual/publ_13676",
    "rdf:type" : [ {
      "@id" : "obo:BFO_0000001"
    }, {
      "@id" : "obo:BFO_0000002"
    }, {
      "@id" : "owl:Thing"
    } ]
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/cite_13676-0",
    "@type" : "thkoeln:Zitat",
    "rdf:type" : {
      "@id" : "owl:Thing"
    }
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/cite_13676-1",
    "@type" : "thkoeln:Zitat",
    "rdf:type" : {
      "@id" : "owl:Thing"
    }
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/cite_13676-2",
    "@type" : "thkoeln:Zitat",
    "rdf:type" : {
      "@id" : "owl:Thing"
    }
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/journal_2283",
    "@type" : [ "bibo:Periodical", "bibo:Journal", "bibo:Collection", "thkoeln:Zeitschrift" ],
    "publicationVenueFor" : "https://fis.th-koeln.de/vivo/individual/publ_13676",
    "rdf:type" : [ {
      "@id" : "obo:IAO_0000030"
    }, {
      "@id" : "obo:BFO_0000031"
    }, {
      "@id" : "obo:BFO_0000001"
    }, {
      "@id" : "obo:BFO_0000002"
    }, {
      "@id" : "owl:Thing"
    } ],
    "label" : "Frontiers in Bioengineering and Biotechnology"
  }, {
    "@id" : "https://fis.th-koeln.de/vivo/individual/publ_13676",
    "@type" : "foaf:Document",
    "artikelnummer" : "1076524",
    "dokumententyp" : "Wissenschaftlicher Artikel",
    "peerReviewed" : "true",
    "sprache" : "englisch",
    "status" : "validiert",
    "hatForschungsfeld" : "https://fis.th-koeln.de/vivo/individual/FF_001",
    "hatOrganisationseinheit" : "https://fis.th-koeln.de/vivo/individual/10000159",
    "hatRessourceart" : "http://cris.nrw/hisinone/RessourceText",
    "hatZitat" : [ "https://fis.th-koeln.de/vivo/individual/cite_13676-1", "https://fis.th-koeln.de/vivo/individual/cite_13676-2", "https://fis.th-koeln.de/vivo/individual/cite_13676-0" ],
    "hatZugangsrecht" : "http://cris.nrw/hisinone/OpenAccess",
    "date" : "2026-04-04T01:09:29",
    "publisher" : "https://fis.th-koeln.de/vivo",
    "rights" : "https://fis.th-koeln.de/vivo/termsOfUse",
    "abstract" : "To date, the establishment of high-titer stable viral packaging cells (VPCs) at large scale for gene therapeutic applications is very time- and cost-intensive. Here we report the establishment of three human suspension 293-F-derived ecotropic MLV-based VPCs. The classic stable transfection of an EGFP-expressing transfer vector resulted in a polyclonal VPC pool that facilitated cultivation in shake flasks of 100 mL volumes and yielded high functional titers of more than 1 × 10⁶ transducing units/mL (TU/mL). When the transfer vector was flanked by transposon terminal inverted repeats (TIRs) and upon co-transfection of a plasmid encoding for the transposase, productivities could be slightly elevated to more than 3 × 10⁶ TU/mL. In contrast and using mRNA encoding for the transposase, as a proof of concept, productivities were drastically improved by more than ten-fold exceeding 5 × 10⁷ TU/mL. In addition, these VPC pools were generated within only 3 weeks. The production volume was successfully scaled up to 500 mL employing a stirred-tank bioreactor (STR). We anticipate that the stable transposition of transfer vectors employing transposase transcripts will be of utility for the future establishment of high-yield VPCs producing pseudotype vector particles with a broader host tropism on a large scale.",
    "doi" : "10.3389/fbioe.2023.1076524",
    "volume" : "11",
    "mostSpecificType" : "thkoeln:Journalartikel",
    "dateTimeValue" : "http://cris.nrw/date2023",
    "freetextKeyword" : [ "mRNA transfection", "murine leukemia virus (MLV)", "gene therapy", "retroviral vector", "stirred-tank bioreactor", "sleeping beauty transposon", "suspension cell" ],
    "hasPublicationVenue" : "https://fis.th-koeln.de/vivo/individual/journal_2283",
    "relatedBy" : [ "https://fis.th-koeln.de/vivo/individual/aut_13676-3", "https://fis.th-koeln.de/vivo/individual/aut_13676-7", "https://fis.th-koeln.de/vivo/individual/aut_13676-2", "https://fis.th-koeln.de/vivo/individual/aut_13676-1", "https://fis.th-koeln.de/vivo/individual/aut_13676-6", "https://fis.th-koeln.de/vivo/individual/aut_13676-8", "https://fis.th-koeln.de/vivo/individual/aut_13676-5", "https://fis.th-koeln.de/vivo/individual/aut_13676-4" ],
    "rdf:type" : [ {
      "@id" : "owl:Thing"
    }, {
      "@id" : "obo:BFO_0000001"
    }, {
      "@id" : "obo:IAO_0000030"
    }, {
      "@id" : "obo:BFO_0000031"
    }, {
      "@id" : "bibo:Document"
    }, {
      "@id" : "thkoeln:Artikel"
    }, {
      "@id" : "obo:BFO_0000002"
    }, {
      "@id" : "thkoeln:Journalartikel"
    }, {
      "@id" : "thkoeln:Publikation"
    } ],
    "label" : [ "RDF description of Novel Suspension Retroviral Packaging Cells Generated by Transposition Using Transposase Encoding mRNA Advance Vector Yields and Enable Production in Bioreactors - https://fis.th-koeln.de/vivo/individual/publ_13676", "Novel Suspension Retroviral Packaging Cells Generated by Transposition Using Transposase Encoding mRNA Advance Vector Yields and Enable Production in Bioreactors" ]
  } ],
  "@context" : {
    "relates" : {
      "@id" : "http://vivoweb.org/ontology/core#relates",
      "@type" : "@id"
    },
    "label" : {
      "@id" : "http://www.w3.org/2000/01/rdf-schema#label"
    },
    "istOrganisationseinheitVon" : {
      "@id" : "http://cris.nrw/hisinone/istOrganisationseinheitVon",
      "@type" : "@id"
    },
    "hatOrganisationseinheit" : {
      "@id" : "http://cris.nrw/hisinone/hatOrganisationseinheit",
      "@type" : "@id"
    },
    "hatRessourceart" : {
      "@id" : "http://cris.nrw/hisinone/hatRessourceart",
      "@type" : "@id"
    },
    "dateTimeValue" : {
      "@id" : "http://vivoweb.org/ontology/core#dateTimeValue",
      "@type" : "@id"
    },
    "relatedBy" : {
      "@id" : "http://vivoweb.org/ontology/core#relatedBy",
      "@type" : "@id"
    },
    "peerReviewed" : {
      "@id" : "http://cris.nrw/hisinone#peerReviewed"
    },
    "mostSpecificType" : {
      "@id" : "http://vitro.mannlib.cornell.edu/ns/vitro/0.7#mostSpecificType",
      "@type" : "@id"
    },
    "hasPublicationVenue" : {
      "@id" : "http://vivoweb.org/ontology/core#hasPublicationVenue",
      "@type" : "@id"
    },
    "status" : {
      "@id" : "http://cris.nrw/hisinone#status"
    },
    "publisher" : {
      "@id" : "http://purl.org/dc/elements/1.1/publisher",
      "@type" : "@id"
    },
    "freetextKeyword" : {
      "@id" : "http://vivoweb.org/ontology/core#freetextKeyword"
    },
    "rights" : {
      "@id" : "http://purl.org/dc/elements/1.1/rights",
      "@type" : "@id"
    },
    "volume" : {
      "@id" : "http://purl.org/ontology/bibo/volume"
    },
    "artikelnummer" : {
      "@id" : "http://cris.nrw/hisinone#artikelnummer"
    },
    "sprache" : {
      "@id" : "http://cris.nrw/hisinone#sprache"
    },
    "hatZitat" : {
      "@id" : "http://cris.nrw/hisinone/hatZitat",
      "@type" : "@id"
    },
    "doi" : {
      "@id" : "http://purl.org/ontology/bibo/doi"
    },
    "date" : {
      "@id" : "http://purl.org/dc/elements/1.1/date",
      "@type" : "http://www.w3.org/2001/XMLSchema#dateTime"
    },
    "hatZugangsrecht" : {
      "@id" : "http://cris.nrw/hisinone/hatZugangsrecht",
      "@type" : "@id"
    },
    "abstract" : {
      "@id" : "http://purl.org/ontology/bibo/abstract"
    },
    "dokumententyp" : {
      "@id" : "http://cris.nrw/hisinone#dokumententyp"
    },
    "hatForschungsfeld" : {
      "@id" : "http://cris.nrw/hisinone/hatForschungsfeld",
      "@type" : "@id"
    },
    "publicationVenueFor" : {
      "@id" : "http://vivoweb.org/ontology/core#publicationVenueFor",
      "@type" : "@id"
    },
    "istForschungsfeldvon" : {
      "@id" : "http://cris.nrw/hisinone/istForschungsfeldvon",
      "@type" : "@id"
    },
    "ocrer" : "http://purl.org/net/OCRe/research.owl#",
    "owl" : "http://www.w3.org/2002/07/owl#",
    "scires" : "http://vivoweb.org/ontology/scientific-research#",
    "xsd" : "http://www.w3.org/2001/XMLSchema#",
    "skos" : "http://www.w3.org/2004/02/skos/core#",
    "rdfs" : "http://www.w3.org/2000/01/rdf-schema#",
    "ocresd" : "http://purl.org/net/OCRe/study_design.owl#",
    "swo" : "http://www.ebi.ac.uk/efo/swo/",
    "cito" : "http://purl.org/spar/cito/",
    "geo" : "http://aims.fao.org/aos/geopolitical.owl#",
    "ocresst" : "http://purl.org/net/OCRe/statistics.owl#",
    "dcterms" : "http://purl.org/dc/terms/",
    "vivo" : "http://vivoweb.org/ontology/core#",
    "event" : "http://purl.org/NET/c4dm/event.owl#",
    "vann" : "http://purl.org/vocab/vann/",
    "foaf" : "http://xmlns.com/foaf/0.1/",
    "c4o" : "http://purl.org/spar/c4o/",
    "fabio" : "http://purl.org/spar/fabio/",
    "vcard" : "http://www.w3.org/2006/vcard/ns#",
    "thkoeln" : "http://cris.nrw/hisinone#",
    "vitro" : "http://vitro.mannlib.cornell.edu/ns/vitro/0.7#",
    "vitro-public" : "http://vitro.mannlib.cornell.edu/ns/vitro/public#",
    "rdf" : "http://www.w3.org/1999/02/22-rdf-syntax-ns#",
    "ocresp" : "http://purl.org/net/OCRe/study_protocol.owl#",
    "bibo" : "http://purl.org/ontology/bibo/",
    "obo" : "http://purl.obolibrary.org/obo/",
    "ro" : "http://purl.obolibrary.org/obo/ro.owl#"
  }
}
