@prefix ocrer: <http://purl.org/net/OCRe/research.owl#> .
@prefix owl:   <http://www.w3.org/2002/07/owl#> .
@prefix scires: <http://vivoweb.org/ontology/scientific-research#> .
@prefix xsd:   <http://www.w3.org/2001/XMLSchema#> .
@prefix skos:  <http://www.w3.org/2004/02/skos/core#> .
@prefix rdfs:  <http://www.w3.org/2000/01/rdf-schema#> .
@prefix ocresd: <http://purl.org/net/OCRe/study_design.owl#> .
@prefix swo:   <http://www.ebi.ac.uk/efo/swo/> .
@prefix cito:  <http://purl.org/spar/cito/> .
@prefix geo:   <http://aims.fao.org/aos/geopolitical.owl#> .
@prefix ocresst: <http://purl.org/net/OCRe/statistics.owl#> .
@prefix dcterms: <http://purl.org/dc/terms/> .
@prefix vivo:  <http://vivoweb.org/ontology/core#> .
@prefix event: <http://purl.org/NET/c4dm/event.owl#> .
@prefix vann:  <http://purl.org/vocab/vann/> .
@prefix foaf:  <http://xmlns.com/foaf/0.1/> .
@prefix c4o:   <http://purl.org/spar/c4o/> .
@prefix fabio: <http://purl.org/spar/fabio/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix thkoeln: <http://cris.nrw/hisinone#> .
@prefix vitro: <http://vitro.mannlib.cornell.edu/ns/vitro/0.7#> .
@prefix vitro-public: <http://vitro.mannlib.cornell.edu/ns/vitro/public#> .
@prefix rdf:   <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix ocresp: <http://purl.org/net/OCRe/study_protocol.owl#> .
@prefix bibo:  <http://purl.org/ontology/bibo/> .
@prefix obo:   <http://purl.obolibrary.org/obo/> .
@prefix ro:    <http://purl.obolibrary.org/obo/ro.owl#> .

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        a       thkoeln:Zitat , owl:Thing .

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        a       obo:BFO_0000003 , obo:BFO_0000008 , obo:BFO_0000001 , vivo:DateTimeValue , obo:BFO_0000148 , owl:Thing .

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        <http://cris.nrw/hisinone/istOrganisationseinheitVon>
                <https://fis.th-koeln.de/vivo/individual/publ_13438> .

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        a       thkoeln:Zitat , owl:Thing .

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        a       thkoeln:Zitat , owl:Thing .

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        rdfs:label  "Dokument"@de-DE , "Document"@en-US , "Document"@en .

owl:Thing  a    owl:Class .

thkoeln:Journalartikel
        a           owl:Class ;
        rdfs:label  "Journal Article"@en-US , "Journalartikel"@de-DE .

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        a             obo:BFO_0000001 , obo:BFO_0000020 , vivo:Authorship , vivo:Relationship , obo:BFO_0000002 , owl:Thing ;
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<https://fis.th-koeln.de/vivo/individual/publ_13438>
        a                         obo:BFO_0000001 , thkoeln:Publikation , obo:BFO_0000031 , thkoeln:Artikel , obo:IAO_0000030 , foaf:Document , thkoeln:Journalartikel , bibo:Document , obo:BFO_0000002 , owl:Thing ;
        rdfs:label                "Generation of Antibodies Selectively Recognizing Epitopes in a Formaldehyde-Fixed Cell-Surface Antigen Using Virus-like Particle Display and Hybridoma Technology" , "RDF description of Generation of Antibodies Selectively Recognizing Epitopes in a Formaldehyde-Fixed Cell-Surface Antigen Using Virus-like Particle Display and Hybridoma Technology - https://fis.th-koeln.de/vivo/individual/publ_13438" ;
        thkoeln:artikelnummer     "57" ;
        thkoeln:dokumententyp     "Wissenschaftlicher Artikel" ;
        thkoeln:peerReviewed      "true" ;
        thkoeln:sprache           "englisch" ;
        thkoeln:status            "validiert" ;
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                <https://fis.th-koeln.de/vivo/individual/FF_001> ;
        <http://cris.nrw/hisinone/hatOrganisationseinheit>
                <https://fis.th-koeln.de/vivo/individual/10000159> ;
        <http://cris.nrw/hisinone/hatRessourceart>
                <http://cris.nrw/hisinone/RessourceText> ;
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                <https://fis.th-koeln.de/vivo/individual/cite_13438-2> , <https://fis.th-koeln.de/vivo/individual/cite_13438-0> , <https://fis.th-koeln.de/vivo/individual/cite_13438-1> ;
        <http://cris.nrw/hisinone/hatZugangsrecht>
                <http://cris.nrw/hisinone/OpenAccess> ;
        <http://purl.org/dc/elements/1.1/date>
                "2026-04-04T01:33:00"^^xsd:dateTime ;
        <http://purl.org/dc/elements/1.1/publisher>
                <https://fis.th-koeln.de/vivo> ;
        <http://purl.org/dc/elements/1.1/rights>
                <https://fis.th-koeln.de/vivo/termsOfUse> ;
        bibo:abstract             "Efficient induction of target-specific antibodies can be elicited upon immunization with highly immunogenic virus-like particles (VLPs) decorated with desired membrane-anchored target antigens (Ags). However, for example, for diagnostic purposes, monoclonal antibodies (mAbs) are required to enable the histological examination of formaldehyde-fixed paraffin-embedded (FFPE) biopsy tissue samples. Aiming at the generation of FFPE-antigen-specific mAbs and as a proof of concept (POC), we first established a simplified protocol using only formaldehyde and 90 °C heat fixation (FF90) of cells expressing the target Ag nerve growth factor receptor (NGFR). The FF90 procedure was validated using flow cytometric analysis and two mAbs recognizing either the native and FFPE-Ag or exclusively the native Ag. C-terminally truncated NGFR (trNGFR)-displaying native and FF90-treated VLPs derived from HIV-1 did not reveal distinctive changes in particle morphology using transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis. Mice were subsequently repetitively immunized with trNGFR-decorated FF90-VLPs and hybridoma technology was used to establish mAb-producing cell clones. In multiple screening rounds, nine cell clones were identified producing mAbs distinctively recognizing epitopes in FF90- and FFPE-NGFR. This POC of a new methodology should foster the future generation of mAbs selectively targeting FFPE-fixed cell-surface Ags." ;
        bibo:doi                  "10.3390/antib12030057" ;
        bibo:issue                "3" ;
        bibo:volume               "12" ;
        vitro:mostSpecificType    thkoeln:Journalartikel ;
        vivo:dateTimeValue        <http://cris.nrw/date2023> ;
        vivo:freetextKeyword      "antigen display" , "hybridoma technology" , "formaldehyde-fixed paraffin-embedded (FFPE) tissue samples" , "cell-surface antigen" , "virus-like particles" , "antibody discovery" , "nerve growth factor receptor" ;
        vivo:hasPublicationVenue  <https://fis.th-koeln.de/vivo/individual/journal_2247> ;
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        a             obo:BFO_0000001 , obo:BFO_0000020 , vivo:Authorship , vivo:Relationship , obo:BFO_0000002 , owl:Thing ;
        vivo:relates  <https://fis.th-koeln.de/vivo/individual/publ_13438> .

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        a             obo:BFO_0000001 , obo:BFO_0000020 , vivo:Authorship , vivo:Relationship , obo:BFO_0000002 , owl:Thing ;
        vivo:relates  <https://fis.th-koeln.de/vivo/individual/publ_13438> .

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        a             obo:BFO_0000001 , obo:BFO_0000020 , vivo:Authorship , vivo:Relationship , obo:BFO_0000002 , owl:Thing ;
        vivo:relates  <https://fis.th-koeln.de/vivo/individual/publ_13438> .

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        rdfs:label  "Publikationen"@de-DE , "Publications"@en-US .